il 1 β antibody  (Boster Bio)

Journal: Acta Pharmaceutica Sinica. B

Article Title: Dual metabolic-inflammation modulation in MicroRNA@neutrophil-derived microvesicles achieve robust osteoarthritis therapy

doi: 10.1016/j.apsb.2025.09.020

Figure Lengend Snippet: miR140@MVs inhibit the pro-inflammatory effect of LPS-induced BMDM in vitro . (A) The intracellular location of Cy3-miR140@FITC-MVs in BMDM cells stimulated with LPS (100 ng/mL) for 24 h (M1 macrophages). miR140 is labeled with Cy3 (Red) and MVs are stained by FITC-Annexin V (Green). The nuclei were stained by Hoechst 33342 (Blue). Scale bar: 5 μm. (B) Representative flow cytometric analysis of BMDM treated with different formulations. The (C) M1 (CD86 + CD206 – ) and (D) M2 (CD86 − CD206 + ) phenotypes were analyzed by Flowjo. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with M1 group. n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001. (E) Western blotting of TLR4 in LPS-induced BMDM treated with different formulations. (F) The relative expression of the protein band was analyzed by Image J and normalized to its respective baseline controls. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. ∗ P < 0.05. The relative mRNA levels of (G) TNFα , (H) IL6 , (I) IL1β , (J) IL10 , (K) TGFβ in LPS-induced BMDM receiving different formulations. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with the M1 group, n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (L) The TNF α level in LPS-induced BMDM supernatant was detected by ELISA assay. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. No significance, ns; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (F–I) Values are normalized to the M1 (baseline control) group.

Article Snippet: Cytokine concentration in the supernatant was quantified by mouse TNF α or mouse IL1 β enzyme-linked immunosorbent assay (ELISA) kits (Elabscience, USA).

Techniques: In Vitro, Labeling, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Acta Pharmaceutica Sinica. B

Article Title: Dual metabolic-inflammation modulation in MicroRNA@neutrophil-derived microvesicles achieve robust osteoarthritis therapy

doi: 10.1016/j.apsb.2025.09.020

Figure Lengend Snippet: Single miR140@MVs for prolonged OA treatment. (A) Schematic illustration of the administration regimen. (B) The relative miR140 level in the whole-knee joint of DMM mice treated with different formulations for 28 days. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as the control. No significance, ns. (C) Typical images of imageological examinations, histological analysis, TUNEL staining and immunohistochemical analysis. Red arrows: synovial inflammation; Yellow arrows: cartilage defect; Blue arrows: bone marrow edema; Red circles: periarticular osteophytes. M: meniscus; S: synovial; C: cartilage. Scale bars in H&E and safranine O-fast green staining: 100 μm; in TUNEL staining and immunohistochemical analysis: 50 μm. (D) Heatmap of variables of histological scoring in each group. (E) OARSI grades of the mice joints in each group. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as the control. No significance, ns; ∗∗∗∗ P < 0.0001. (F–J) The relative mRNA levels of inflammatory factors in OA joints from mice after different treatments, including (F) TNFα , (G) IL6 , (H) IL1β , (I) IL10 and (J) TGFβ . The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as control. No significance, ns; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001.

Article Snippet: Cytokine concentration in the supernatant was quantified by mouse TNF α or mouse IL1 β enzyme-linked immunosorbent assay (ELISA) kits (Elabscience, USA).

Techniques: Control, TUNEL Assay, Staining, Immunohistochemical staining